-hydroxybenzoic acid (PH) on a nonpolar C18 column subject to the utmost analysis time of six min. The shaded parts represent locations wherever a separation is impossible, Using the unresolved solutes identified.The easiest method to appreciate the theoretical and the practical specifics talked about Within this part is always to carefully analyze

, for example, displays retention periods for four weak acids in two mobile phases with virtually identical values for (P^ prime ). Although the get of elution is the same for equally cell phases, Just about every solute’s retention time is impacted otherwise by the selection of organic and natural solvent.The ultimate way to value the theoretica

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